Tritech Research is pleased to offer a fantastic, complete Dissecting Stereo-microscope System for thousands of dollars less than any competing system.
You are probably used to seeing Stereo-microscopes priced in the $3000-$7000 range. Remarkably, we are offering a system with high-quality optics, equal to the best microscopes, at an unequaled low price! The expert optics and mechanics of our system will let you see all the fine details of your specimens with the utmost clarity. Each system includes all of the following:
This system meets all your dissecting Microscope needs. The magnification changer ring allows you to switch between 6x, 12x, 25x, 50x magnification of the Eyepiece/Objective combination with the turn of your fingers. The lenses are ground and mounted so precisely that it is seldom necessary to re-focus when switching from one magnification to another. Illumination from above is ideal for micro-surgery, dissection, Drosophila and plant genetics, while illumination from below is ideal for Nematode and fungal genetics and micromanipulation.
3 or more @ $2095.00 each
2 @ $2245.00 each
1 @ only $2395.00
Tritech Research has designed a microinjection system that lets you control with exacting precision both the pressure and the duration of the microinjection pulse. And, it's available at a fraction of the cost of competing systems.
Our microINJECTOR System uses a precision pressure regulator and a high-speed electronic solenoid switching valve, yet it is amazingly robust and simple to operate. It allows you to start and stop injection within a fraction of a second and control the quantity and flow of material into your target with precision. Our electronic foot control is designed to keep your hands free so you can concentrate on the manipulation of your needle and target.
The microINJECTOR comes preassembled, ready to use. You need only attach the system to a compressed gas tank (e.g. N2) or in-house pressurized air line at one end and your needle at the other. You set the desired pressure and the system delivers pulses of pressure to your needle. In the base model, the duration of the injection pulse is controlled by how long you hold down the high-speed electronic foot pedal. With our easy-to-use, reliable equipment there is no "magic" required. Even a novice will be turning out transformants the first day.
With our optional electronic Pulse-Length Control Module (PCM) you can set injection times from 0.1 to 10 seconds. Each time the pedal is pressed, a constant volume is reproducibly injected. This option is crucial for quantitative experiments such as transient expression assays following microinjection of DNA, RNA, protein or other substances.
By controlling the pulse length and pressure independently, you can compensate for the flow characteristics of individual needles; you can determine if the needle is properly placed prior to any appreciable damage occurring to your target; and you can fill your target incrementally. High pressure settings with rapid on/off pulses aid in unclogging needles.
As an added option to the microINJECTOR System you can hold your target with our state-of-the art Holding Pipette Controller (HPC)
The HPC allows you to pick-up and hold your target with vacuum suction then release it with positive pressure. Our unique pneumatic foot pedal control allows a continuous range from vacuum through positive pressure depending on the position of the pedal. Ideal for holding and manipulating targets such as embryos and oocytes. Also useful for injecting ES cells into Blastocysts. As any of our customers will attest, within a short time you will be proficiently getting transformants.
$ 425.00: Standard model
$ 75.00: Pulse-length Control Module
$ 275.00: Holding Pipette Controller
$ 50.00: Brass Straight-arm Quick-change Needle Holder
$1,500.00: Narishige Mechanical Joystick-type Micromanipulator
(for the CE-marked Euroversion, add $25.00, and the PCM is required)
Tritech Research's Cloning Gun revolutionizes E. coli transfomation. Finally electroporation is a simple low-cost procedure for every scientist!
The completely portable, hand-held design makes transformation a one-step process. Just plug in one of our revolutionary disposable Pipectrode pipetting electrode tips to The Cloning Gun. It sucks up your cell/DNA mixture, zaps it, and ejects your transformants into the recovery buffer- all in less than 15 seconds! Unlike other systems, our Pipectrode makes the time consuming process of transferring your cell/DNA mixture back and forth with cuvettes and pipettes obsolete.
The Cloning Gun comes factory pre-set to the optimal parameters for E. coli transformation. Transformation efficiency can be greater than 1010 per µg for log phase cells and even stationary phase cells prepared using our revolutionary super-easy protocol featuring our CompBooster Solution. Standard stationary phase cultures can exceed 107 per µg.
The Pipectrode represents a major technical advance to electroporation. The most important factors for highest transformation efficiency are low toxicity, temperature control of the sample during electroporation and rapid recovery afterward.
Thin surgical stainless steel electrodes backed by water-ice temperature stabilizers provide a superior environment for cells during electroporation. We make use of the "latent heat of fusion" of water (it takes the same number of calories to melt 1g of ice as to heat 1g of water 80°C!), so your sample stays cool, which is also important for no arcing. Since the Pipectrode is a pipet, you can eject your sample into recovery buffer instantly.
Only $ 1.50 each!* If you prepare your own cells, you save a lot of media and a lot of time using The Cloning Gun with stationary phase cells.
Comparison Costs for 100 Transformations (>2 x 109/µg)** Company "Cuvettes" Cells* Supply Total Time in batches of 5 BioRad 2 x $135 10 x $99 $1260 200 min. BRL 3 x $70 10 x $150 $1710 300 min. BTX 2 x $126 none available $1800? 200 min. Invitrogen 2 x $125 10 x $175 $1900 200 min. Stratagene 2 x $135 10 x $135 $1620 200 min. Strategene "Ultra Comp." 10 x $145 $1450 700 min. and a world apart... Cloning Gun 2 x $75 5 x $50 $400 <17 min.
** Based on recent catalogue prices; approximate times in our lab.
microMAGNIPULATOR
AND STRAIGHT ARM NEEDLE HOLDER
At last there is a versatile micromanipulator for under $1000! The microMAGNIPULATOR uses the same principle as the most expensive microscope glide stages to allow you to accurately position your needle for microinjection. The surfaces of both the sliding needle positioner and glide base are teflon coated for smooth movement, and held together by a powerful, adjustable magnet capable of pulling with over 100 pounds of force. The glide base is a 4" solid steel cube that weighs 20 pounds. Its mass and no-slip elastomeric feet help to dampen vibrations. X-Y control in the 10 micron range is accomplished either by pushing the sliding needle positioner directly with your hand, or via threaded push bolts. Z control is accomplished using a fine threaded adjustment knob. Over 3 cm of X and Y movement and over 1 cm of Z movement are possible. The verstile needle positioning stand can be user-configured in a variety of ways to hold your needle at the height and angle you prefer.
And, what goes between the Tritech Research microINJECTOR and microMAGNIPULATOR (or anybody else's microinjection/micromanipulation system, for that matter)? You need a high-quality Straight-arm Needle Holder to couple the pressure to your glass capillary needle, and the needle to the micromanipulator. Now you don't have to pay $150 for Leitz-style compression fitting Needle Holders, because you can get our solid brass, silicone rubber compression fitting Needle Holder!
$ 995.00: microMAGNIPULATOR gliding needle positioning system
$ 50.00: Top-quality brass needle holder
