GELSTOPPER
(U.S. pat. no. 5,120,419)
Never worry about your gels running too far again! Be carefree with Tritech Research's GelStopper System.
The Tritech Research GelStopper has a micro-sensor that is almost as sensitive as the human eye! You position the micro-sensor behind the gel where you wish the marker-dye to stop. When your gel is done, the GelStopper turns off your gel, sounds an alarm (or both). It can even call you at home if you have the right computer and modem set-up...
The GelStopper works with all visible dyes, with horizontal and vertical DNA sequencing and protein gels. You only need a clear electrophoresis system that can transmit light to the sensors through the gel.
Special circuitry allows it to stop your gel using the first or second marker band. Ideal for long DNA gels when the Bromophenol Blue should run off and the gel should stop when the Xylene Cyanol reaches the sensor.
$ 169.00
Size-Select and Concentrate your DNA or RNA right in your Gel!
Recovering intact nucleic acids from a gel is simplified with our special formulation of Super-Agarose.
Once you have run your gel, cut a small trough in front of your band(s) of interest, fill it in with our ready made Super-Agarose, and electrophorese in the nucleic acid. Super-Agarose's formulation decreases the DNA's electrophoretic mobility to near zero; thus messy broad bands, and even multiple bands, "pile-up" and are compressed into a minimum volume of agarose (fig. 1). That means you get all of your nucleic acid in a few microliters of high-quality, low-melting temperature gel, rather than in a few hundred microliters. Plus there is no loss or shearing of high molecular weight molecules since they remain in the gel.
Use Super-Agarose to obtain preparative amounts of DNA or RNA from minor bands, or of size-selected genomic DNA (fig. 2). Run your gel as usual, loading several adjacent lanes with the same sample of interest; cut out the appropriate band across those lanes; cut another perpendicular hole, slightly longer than your band slice, elsewhere in the gel (or in a new gel). Place your band slice in the new hole and fill the extra space (toward the anode) with Super-Agarose (fig.2). Your nucleic acid will run until it becomes highly concentrated within the Super-Agarose: ready for your demanding application! (fig.2) Virtually all enzymatic reactions can be performed "in gelo" or you can extract if you wish.
Every batch of Super-Agarose is tested and guaranteed to be free of nuclease, protease, and "enzyme" inhibitors.
Figure1.
a) DNA from .8-2kb concentrated on Super-Agarose
b) Same sample, no Super-Agarose
Figure
2
Protocol for preparative "in gelo" concentration of DNA or RNA (see
text for description)
2 ml: $ 9.00 (10-40 gels)
12ml: $36.00 (60-240 gels)
25 ml: $49.00 (120-480 gels)
Automatic Marker Entry!
GELS 3.0 is an amazing time saving innovation - simply tape a photo
or autoradiogram of your gel to your Macintosh screen and the program prompts
you to click in your marker bands using the mouse and cursor! Use the Markers
Menu to select any of the commonly used DNA and protein marker sets and
the program will automatically prompt you to enter each band in the set
by clicking on them, or you can select Other Markers and enter your own
Band Input
Click on your experimental bands lane by lane. Each time a band is
clicked, its Molecular Weight is calculated automatically, based on the
markers you entered, and instantly displayed, along with the total Molecular
Weight of all bands in that lane. Simultaneously, GELS 3.0 displays a mock
gel on the screen.
Data Output
Select Preview Bands and GELS 3.0 scales your data to produce a clear
full-screen image of the mock gel. Select Preview Sizes and each band in
the mock gel is replaced by the text of its Molecular Weight! Print Bands
and Print Sizes optimize the output for your printer and produce beautiful
data for your notebook or group meeting discussions.
Merging Data
The merge Data command lets you compare experiments performed on separate
gels, with separate size markers, or a small photo of a gel with a life-sized
autoradiogram of that same gel, side by side, so that bands can be identified
easily.
Editing Data
Full Cut-and-Paste editing of the bands and lanes in the mock gel is
supported so that you can experiment with possible doublets and faint bands
until a logical consistent restriction map emerges.
If nucleic acids are not your favorite molecules, GELS 3.0 is just as useful for dealing with proteins, carbohydrates, or any other molecules you run through a sizing gel. 50 lanes of information can be stored at once (with 50 bands per lane). It is difficult to list all the features of Gels 3.0, but easy to use them. Full on-line help and documentation are provided (though seldom needed!)
$ 49.95
Design Oligo-Nucleotide Probes in about 1 minute!
Oligo-Designer 1.0 maximizes your chances of cloning genes by protein sequence homology by helping you choose probes with the lowest degeneracy. It takes your protein sequence (which you can type in, or cut and paste from anywhere) and Reverse-Translates it for you. The key is in the graphic form of the output: the degenerate nucleotide code "hangs down" from the amino-acid sequence. This makes finding the least ambiguous sequences quick and easy - just look for indentations (contiguous areas where the least is hanging down) and you have your probe sequences, ready to synthesize and clone your gene!!! It's an almost instantaneous process.
$ 30.00
