Microinjection Tips Index:

• Hot Tip 1:

Worms can be injected under mineral oil instead of halocarbon oil.
• Hot Tip 2:

Where to get Halocarbon Oil

Hot Tip 1

Several more people confirm that mineral oil is the way to go. So, save money and use it instead. I want to start putting a list of money-saving tips like this into our new website, which is, for the time being, at:

http://members.aol.com/TriResInc (Yes, I am embarrassed, and yes, AOL sucks)

So, please contribute other tips. There is a way to do that from the site, too. Here is more support for using the mineral oil instead of halocarbon...

                                                                                                                                   Andy

info@TritechResearch.com

 

1.1
I used halocarbon oil in Boulder and Mineral oil when I moved to SF. It's all we use now.

Craig P. Hunter

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1.2
Hi Andy,
I've been using light mineral oil from Sigma for my micro-injections with reasonable results. I'm not a great micro-injector, so I can't say that it works just as well as halocarbon oil or not, but I've been able to get transgenics with no obvious problems from the oil. I'm pretty good at killing worms, though...

John Lye

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1.3
Andy- Of course the dechorionated fly eggs are stuck onto doublestick tape and submerged in the halocarbon oil for 24 during development- we rescue our adult worms immediately...

Anne C. Hart

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1.4
Hi ANdy-
We use mineral oil as well. Worms dry out more quickly with mineral oil, but I prefer it: not as messy to work with. To deal with the drying out problem, I tend to inject one worm at a time, but have found that I can inject as quickly as people lining up multiple worms on a slide. And the worms survive better, seem healthier if I only inject one at a time.
All the best, Susan

Susan Mango

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1.5
Hi Andy,
Our lab has always used mineral oil (Sigma) for injections. I can't remember off-hand if it is heavy or light (I think heavy).
Paul

Paul Muhlrad

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Hot Tip 2

2.1
According to a WWW page on microinjecting worms by Michael Koelle (I don't have the WWW address handy) Halocarbon Oil Series 700 (CAS #9002-83-9) is available from

Halocarbon Products Corporation,
PO Box 661, River Edge, NJ 07661; Phone: 803-278-3500

Bill

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2.2
Hi Andy--
We get our halocarbon oil from

Halocarbon Products
PO Box 661 River Edge, N.J. 07661
800-338-5803
Series 700 halocarbon oil, 1 lb, (1995 price $ 84.00)
FOB N. Augusta, S.C.

it works great for us.
cheers,

Wendy Katz

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2.3
Andy-
I've always used SIGMA catalog # 400-5, Heavy white mineral oil. Last time I bought it, $3.70 for 100 ml bottle (nearly a life-time supply).

Curtis M. Loer

Dept. of Biology

University of San Diego

5998 Alcala Park

San Diego, CA 92110

619-260-4129 FAX: 260-6804

cloer@acusd.edu

http://www.acusd.edu/~cloer/

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2.4
Andy
The halocarbon oil from Sigma is just right for us. The catalogue number is H-8898 (Halocarbon oil 700).

Neville Ashcroft

ABL-Basic Research Program

NCI-FCRF, Frederick, MD 21702

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2.5
hi.
we use halocarbon oil 7000 from Sigma H-8898 seems to work fine for us.
take care,

andy golden

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2.6
Reply to:RE>halocarbon oil source?

We purchase halocarbon oil, series 700 in 1 lb. bottles from Halocarbon Products Corp., 120 Ditmann Ct., N. Augusta, SC 29841. The cost is $70.00/lb.

Cathy Branda,

Stern Lab, Yale University

 

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